Fig. 6: Biosynthesis and regulation of phthalides and other terpenoid volatiles in Angelica roots.

a Headspace solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis of the contents and composition of volatiles in Angelica roots from early-flowering (EF) and normally growing (NG) plants. b Differential content analysis of the volatiles in Angelica roots between EF and NG plants. c Enzymatic reactions in the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways in plants and synthesis of short-chain prenyl diphosphates. The MVA pathway is shown in light red; the MEP pathway is shown in light green. Abbreviations and full names are given in Table S16. Data are expressed as the means ± SDs from at least three independent experiments with triplicates. Differences between NG and EF samples are considered significant when **P < 0.01 and *P < 0.05 in Student’s t test.