Fig. 2: Localization of T. gondii tachyzoite clusters in the fetal guinea pig brain. | Communications Biology

Fig. 2: Localization of T. gondii tachyzoite clusters in the fetal guinea pig brain.

From: Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii

Fig. 2

a DAPI staining (blue) and immunofluorescence for SAG1 (red) on a 30 µm brain section of a guinea pig fetus of a dam inoculated with T. gondii on gestation day 23 and dissected on day 48. Open arrowheads indicate a SAG1+ T. gondii tachyzoite cluster. Scale bar, 10 µm. b Number of tachyzoite clusters detected by SAG1 immunofluorescence in the distinct brain regions of guinea pig fetuses of dams inoculated with T. gondii on gestation day 23 and dissected on days 40 and 48. Data are from all guinea pig fetal brains with Ct value below 35 as determined by qPCR for T. gondii (n = 5 biologically independent samples for gestation day 40, n = 10 biologically independent samples for gestation day 48, Supplementary Table 1). c Median section of an adult guinea pig brain. NC neocortex, LV lateral ventricle, BN basal nuclei, SA septal area, T thalamus, HT hypothalamus.

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