Fig. 6: GFAP expression in the fetal guinea pig neocortex. | Communications Biology

Fig. 6: GFAP expression in the fetal guinea pig neocortex.

From: Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii

Fig. 6

a, b DAPI staining (blue) and immunofluorescence for GFAP (green) on 30 µm brain cryosections of guinea pig fetuses of control (a) or infected (b) dams dissected on gestation day 48. Dams were inoculated with T. gondii on gestation day 23. Scale bar, 50 µm. The entire cortical wall is shown. CP, cortical plate. Scale bars, 50 µm. c Quantification of GFAP+ labeling intensity in the cortical wall, expressed as number of voxels per 100 µm ventricular surface. Cortical wall corresponding to a total ventricular surface of 150–250 µm was analyzed. Center line, mean; error bar, SEM. Data of the infection groups are from all guinea pig brains with Ct value below 35 as determined by qPCR for T. gondii (n = 10 biologically independent samples, for gestation day 48, Supplementary Table 1). Data of the control group are as follows: n = 5 biologically independent samples. (P = 0.3325, df = 13).

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