Fig. 8: Optogenetic activation of the ACC-ACC induced pain behavior in the contralateral hind paw in nerve-injured mice, whereas inhibition relieved pain. | Communications Biology

Fig. 8: Optogenetic activation of the ACC-ACC induced pain behavior in the contralateral hind paw in nerve-injured mice, whereas inhibition relieved pain.

From: Activation of the glutamatergic cingulate cortical-cortical connection facilitates pain in adult mice

Fig. 8

a A schematic diagram showing the placement of the virus injected in one side ACC and the optic fiber implantation in the contralateral ACC. b Yellow light inhibition of the ACC-ACC significantly increased the right hind paw withdrawal threshold (n = 5 mice, p < 0.05 for BL versus eNpHR of the right hind paw). c Yellow light inhibition of the ACC-ACC did not affect the response latency of the hot plate test in mice (n = 5 mice). d Experimental timeline of the nerve injury and behavior tests in mice. e Compared with the sham group, the mechanical threshold of the ipsilateral hind paw with injury was significantly reduced in the nerve injury group (Sham, n = 8 mice; Nerve injury, n = 12, for Sham versus Nerve injury, p < 0.001 for 3 d, 7 d, 14 d, and 28 d). f Blue light evoked-activation of the ACC-ACC after nerve injury did not affect the withdrawal threshold of the ipsilateral hind paw (n = 7 mice). g Yellow light evoked-inhibition of the ACC-ACC significantly increased the withdrawal threshold of the ipsilateral hind paw after nerve-injured in mice (n = 5 mice, for Nerve injury versus Nerve injury + eNpHR, p < 0.01 for 3 d, p < 0.05 for 7 d, p < 0.01 for 14 d, p < 0.001 for 28 d). h Compared with the sham group, the mechanical threshold of the contralateral hind paw with injury was significantly reduced in the nerve injury group (Sham, n = 8 mice; Nerve injury, n = 12 mice, for Sham versus Nerve injury, p < 0.01 for 3 d and 28 d, p < 0.001 for 7 d and 14 d). i Blue light activation of ACC-ACC significantly decreased contralateral hind paw withdrawal threshold 3, and 14 days after nerve-injured in mice (n = 7 mice, for Nerve injury versus Nerve injury +ChR2, p < 0.05 for 3 d, p < 0.01 for 14 d). j Yellow light inhibition of ACC-ACC significantly increased the withdrawal threshold of the contralateral hind paw 7 days after nerve-injured in mice (n = 5 mice, p < 0.01 for Nerve injury versus Nerve injury +eNpHR of 7 d). Error bars represent SEM, *indicates significant difference (*p < 0.05, **p < 0.01, ***p < 0.001). ACC anterior cingulate cortex, BL baseline.

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