Fig. 2: Single-molecule fluorescence histograms compiled from collections of individual molecules of A2AAR, A2AAR[D52N], A2AAR[I92N] and A2AAR[R291Q]. | Communications Biology

Fig. 2: Single-molecule fluorescence histograms compiled from collections of individual molecules of A2AAR, A2AAR[D52N], A2AAR[I92N] and A2AAR[R291Q].

From: Single-molecule visualization of human A2A adenosine receptor activation by a G protein and constitutively activating mutations

Fig. 2

a Histograms compiled for A2AAR with no ligand added (apo), in complex with the antagonist ZM241385, and in complex with the agonist NECA. The dashed lines represent fits to Gaussian populations centered around the normalized intensities of 1 (black), 2 (blue), and 3 (red). The composite fit representing the sum of the Gaussian fitted curves is shown as a solid black line. Error bars represent the standard error calculated for each bar of the histogram. b–d Histograms compiled for b A2AAR[D52N], c A2AAR[I92N] and d A2AAR[R291Q]. Same presentation details as in (a).

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