Table 1 Sequencing and assembly results of fish and plant samples from extended storage conditions.

From: Generating high-quality plant and fish reference genomes from field-collected specimens by optimizing preservation

 

Storage

Sequencing

Assembly

Spp.

Solv

°C

wks

N50.s

N50.d

Long

Q_avg

cov(x)

N50

contig

C(%)

QV.n

QV.hy

methy

Mcal

ET

22

6

3.4

9.8

176.5

18.7

58

6.5

1761

98.7

36.2

37.9

NaN

Gnig

ET

22

6

3.5

15.2

139.4

17.9

37.8

9.7

1441

98.5

38.0

40.1

NaN

Kazu

LN

−80

0

16.9

54.4

418.9

20.1

28.4

21.8

641

98.8

40.2

42.0

79.14

Kazu

ET

22

6

15.8

20.5

183.4

19.1

33.9

13.8

792

98.6

40.3

42.4

78.66

Kazu

RN

22

6

13.7

15.1

225.5

19

11.2

5.1

795

98.7

39.9

42.3

80.33

Mesc

LN

−80

0

21.6

38.7

367.8

19.2

52.8

16.5

460

99

47.5

49.3

77.63

Mesc

ET

4

3

13.9

24.1

296.2

17.8

58

10.4

638

99.2

43.8

44.6

77.26

Mesc

RN

22

3

1.9

6.2

392.3

17.2

37.3

0.4

18881

99

32.1

32.5

75.55

Sbic

ET

4

3

9.1

12.7

394.9

16

82.9

5.9

1705

94.6

44.3

46.4

NaN

  1. Spp: fish and plant species M. californiensis (Mcal), G. nigericans (Gnig), K. azureus (Kazu), M. esculenta (Mesc), S. bicolor (Sbic).
  2. Solv: solvent used ET (95% EtOH), RN (RNAlater), LN (Liquid Nitrogen control).
  3. N50.s: sequencing read N50 (kb) from shallow seq approach (NanoPlot) of untreated DNA.
  4. N50.d: sequencing read N50 (kb) from deep seq approach (NanoPlot) of DNA processed through SRT.
  5. Long: longest read observed in sequencing (kb).
  6. Q_avg: mean quality score of library (NanoPlot).
  7. cov(x): estimated sequencing coverage from ONT.
  8. N50: the assembly N50 (Mb).
  9. contig: total number of contigs from assembly.
  10. C(%): BUSCO completeness.
  11. QV determined using Merqury: QV.n from ONT only polishing with ‘Racon’ ; QV.hy from ONT and Illumina polishing with ‘Pilon’.
  12. methyl: % of Cytosine bases which are methylated as determined from ONT dorado basecaller.
  13. NaN: refers to not performed.