Fig. 1: Detection of Akaluc/AkaLumine bioluminescence by oral administration of AkaLumine in Drosophila.

a Schematic diagram of the luminescence measurements. Each well of the 24-well plate contained a single fly and food containing AkaLumine. The luminescence emitted from the fly freely moving in the well was measured by the upper and lower bioluminescence detectors. b Luminescence was detected by administering AkaLumine to flies expressing Akaluc. Akaluc flies (tubP-Gal4 > UAS-Venus-Akaluc) or wild-type flies (w¹¹¹⁸) were used. In AkaLumine administration groups, flies were fed food with AkaLumine (1.0 mM) for 24 h prior to the start of luminescence measurements, and measurements were performed continuously for 24 h. The group not administered AkaLumine was fed a normal diet and measurements were taken. The graph shows the mean value of luminescence measured continuously for 24 h in each trial. The number of trials for each data set is shown on the graph. One-way ANOVA with Tukey-Kramer test was used for a statistical test. ****p < 0.0001, ns no significance. Error bars indicate s.e.m. c The examination of appropriate AkaLumine concentrations for in vivo monitoring of Akaluc/AkaLumine bioluminescence. Akaluc flies (tubP-Gal4 > UAS-Venus-Akaluc) were orally administered each concentration of AkaLumine 24 h prior to the start of luminescence measurements, and measurements were performed continuously for 24 h. The group with zero AkaLumine was fed normal food. The graph shows the mean value of luminescence measured continuously for 24 h in each trial. The number of trials for each data set is shown in the graph. Error bars indicate s.e.m. d The temporal changes of bioluminescence after the administration or withdrawal of AkaLumine food. Luminescence measurements were started as soon as AkaLumine was administered to Akaluc-expressing flies (red arrows). At 48 h after the start of measurements, the flies were transferred to wells with normal food without AkaLumine under cold anesthesia (blue arrows), and bioluminescence measurements were carried out for an additional 24 h. The darker colored line in each graph indicates the mean and the lighter colored line indicates the error bars (s.e.m). The number of trials for each graph is n = 6.