Fig. 4: 3D imaging of fixed endoplasmic reticulum with F-VCD.
From: Video-rate 3D imaging of living cells using Fourier view-channel-depth light field microscopy
a Comparison of deconvolution-based reconstruction and F-VCD reconstruction. The magnified images of two selected ROIs show a significant quality difference between the two reconstruction approaches. b The y–z section plane of deconvolution results and F-VCD results along the white dash line in (a). The frequency spectrums of the axial planes show the spatial resolution enhancement offered by F-VCD. The white frames indicate that F-VCD outperforms in artifact suppression. The box plots show the statistics results of resolution quantification of the deconvolution approach (lateral: 509.0 ± 67.5 nm, axial: 704.6 ± 70.2 nm; p-value: 2.82 × 10−8) and F-VCD (lateral: 202.0 ± 23.4 nm, axial: 480.9 ± 37.2 nm; p-value: 9.99 × 10−4).
