Fig. 5: Imaging and analyzing mitochondrial dynamics in living cells using F-VCD enhanced FLFM.
From: Video-rate 3D imaging of living cells using Fourier view-channel-depth light field microscopy
a Left: raw LF image at T = 0 s; Right: comparative deconvolution and F-VCD reconstructions of captured LF. b The magnified views of the red rectangular region shown in (a), demonstrate the enhancement of F-VCD across the entire acquisition time of 3 min. c Fast morphological dynamics of mitochondria. Top panel: captured LF views; Bottom panel: the MIPs of F-VCD reconstruction. d 3D tracking of mitochondrial fusion, fission, and tubulation. e Representative images related to the three types of mitochondria events shown in (d). Scale bar:1 μm. f Velocity changes during the occurrence of the three mitochondrial events. g Mitochondrial velocity as a function of distance during the occurrence of the three events.
