Fig. 5: The starch/AMG hydrogels are more effective than glucose hydrogels in increasing the in vivo proangiogenic functions of hMSCs after subcutaneous implantation.
From: Enzyme-controlled, nutritive hydrogel for mesenchymal stromal cell survival and paracrine functions
a A gross view of the hydrogel and schematic (not to scale) of the experimental model designed to assess the proangiogenic potential of hMSC-containing hydrogels. (Created with BioRender.com). b, c Micro-CT vasculature analysis in the vicinity of either glucose-free, 5.5 mM glucose, or starch 2%/AMG hydrogels after 14 and 21 days of ectopic implantation in mice. b Representative 3D reconstructions of the new blood vessels into the volume of interest (VOI) of either glucose-free, 5.5 mM glucose, or starch 2%/AMG hydrogels after subcutaneous implantation in nude mice, scale bars: 500 µm. c Quantification of new blood vessel volume into the VOIs of either glucose-free, 5.5 mM glucose, or starch 2%/AMG hydrogels after subcutaneous implantation in nude mice (n = 8). d Table summarizing the number, diameter, and length of newly formed blood vessels into the VOIs of either glucose-free, 5.5 mM glucose, or starch 2%/AMG hydrogels after subcutaneous implantation in nude mice (n = 8).
