Fig. 5: AKT inhibition could reverse the immune-suppressive phenotype of the EMThigh-AKT subtype in vivo. | Communications Biology

Fig. 5: AKT inhibition could reverse the immune-suppressive phenotype of the EMThigh-AKT subtype in vivo.

From: Tumor microenvironment remodeling plus immunotherapy could be used in mesenchymal-like tumor with high tumor residual and drug resistant rate

Fig. 5: AKT inhibition could reverse the immune-suppressive phenotype of the EMThigh-AKT subtype in vivo.

a EMT classification of mGSC, 4T1 and B16-F10 cell line using machine learning method. b Flowchart of CyTOF Analysis. c Comparison of CD45+ immune cell’s percentage between NC group and MK2206 group. d 20 clusters were acquired in CD45+ immune cells. e TAM’s infiltration has a tendency to decrease in MK2206 group compared to NC group. f IA_IE expression is upregulated in TAM’s of MK2206 group. g CD163 expression is downregulated in TAM’s of MK2206 group. h PDL1 expression is downregulated in TAM’s of MK2206 group. i T cell’s infiltration increased in MK2206 group compared to NC group. j Expression heatmap of T cell’s function markers. k T cell’s dysfunction status was reversed by MK2206 without increase of memory T cells. l Mesenchymal transition status did not alter after MK2206 inhibition. Error bars indicate SD; the Student’s t test was used to analyze statistical significance between 2 groups; In NC group, n = 3 biological replicates; In MK2206 group, n = 4 biological replicates.

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