Fig. 5: Schematic representation of the software tools performance.

a Graphical representation of the software distribution in terms of the annotated lipid species. The bars display the percentage of lipid species within each lipid class annotated by four (light green), three (pink), two (blue), or one (orange) software. Finally, the dark green color corresponds to lipids annotated by manual chromatogram and MS/MS spectra inspection. The figures from (b–e) represent the lipid species annotated by each software tool (Lipid Annotator, (b); MS-Dial, (c); LipidHunter, (d); LipidMS, (e)).