Fig. 2: Peptide sequences selected for tissue-specific assay development.

The properties of the 2965 proteotypic surrogate peptides selected for MRM assay development. a peptide length b peptide retention time in minutes (teal bars) compared to percentage of organic buffer B (red line) in the gradient, where buffer B consists of 0.1% formic acid in acetonitrile c hydrophobicity as indicated by GRAVY score d precursor ion charge e identity of the top 5 most intense ions. Ion series type is shown by yellow (y ions) or tan (b ions).