Fig. 9: Mass spectrometry analysis of σE binding partners.
From: σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
a Pulldown of σE-HA (LK3802 strain) in non-stressed conditions (equal to time 0 min in western blot experiment in Fig. 1). The same pull-down experiment was performed with the wt strain (LK3801) as a negative control and obtained data were used as a background (gray dots). Thresholds (dashed lines) were set-up as 1.3 and 1.5 for fold-change (x-axis) and Significance (y-axis), respectively. Significantly enriched ribosomal proteins (red dots), unknown proteins (blue dots), and transcription or DNA binding proteins (green dots) are highlighted. b Pulldown of σE-HA (LK3802 strain) in EtOH-stressed conditions (equal to time 60 min in western blot experiment). The same pull-down experiment was performed with the wt (LK3801) strain as a negative control and obtained data were used as a background (gray dots). Proteins are highlighted as in chart a.
