Fig. 5: CD3+CD19+ cells in vaccinated mice responded to antigen-specific stimulation.
From: A biphenotypic lymphocyte subset displays both T- and B-cell functionalities
a Female C57BL/6 J mice were immunized intramuscularly with a DNA vaccine encoding SARS-CoV-2 S1 protein for three times at an interval of 2 weeks. Four weeks post the third vaccination, the mice were euthanized and splenocytes were collected for assays of antigen-specific cellular immune responses. b Representative plots for FACS gating of S1 protein-specific cytokine-secreting cells (IFN-γ, IL-2, or TFN-α). c The frequencies of S1 protein specific IFN-γ, IL-2 and TFN-α secreting cells in different subsets (mean ± SD, n = 6). d Representative plots for FACS gating of S1 protein binding cells. e The frequencies of S1 specific B cells in different subsets (mean ± SD, n = 5). All error bars represent SD. Statistical analyses were performed using the method of t-test.
