Fig. 2: Nucleotide content of the crystallized tetramer. | Communications Biology

Fig. 2: Nucleotide content of the crystallized tetramer.

From: The structure of a tetrameric septin complex reveals a hydrophobic element essential for NC-interface integrity

Fig. 2

A Polder-OMIT maps (contoured at 4.0 σ) show that all subunits contain GDP. B The chromatogram of a representative analytical IEX chromatography reveals the presence of mainly GDP. The inset shows a Coomassie stained SDS-PAGE gel of the purified complex which was subsequently denatured for nucleotide content determination. C Integration of the peaks from the IEX chromatograms (N = 4, error bars represent SD) confirms that the complex contains 87% GDP. The remaining GTP originates likely from a mixed population in one of the subunits.

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