Fig. 3: Features of the G-interfaces. | Communications Biology

Fig. 3: Features of the G-interfaces.

From: The structure of a tetrameric septin complex reveals a hydrophobic element essential for NC-interface integrity

Fig. 3

A Coordination of the β-phosphate by residues from the P-loop, shown exemplarily for Cdc10. B Triangular arrangement by Shs1K36 and Shs1K52 with the β-phosphate. Another triangular arrangement is formed at the α-phosphate in Shs1 by Shs1H51 and Cdc12H155 from the neighboring subunit. C The exceptionally long switch 1 loop (colored magenta) of Shs1 snuggling along the surface of the Shs1 subunit. D The highly conserved Arg(βb) is the central element of a conserved interaction hub in the nucleotide-binding pocket, shown here for Cdc3R360. Arg(βb) is coordinated by Cdc3D289 and sandwiches together with Cdc3K287 the guanine ring in a π-cation stack. Arg(βb) connects furthermore the G4 motif to a conserved Glu(α4) of the neighboring subunit (here Cdc10E188), maintaining a stabilizing inter-domain contact. Dotted lines in A, B and C indicate hydrogen bonds.

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