Fig. 4: AlphaFold assisted assembly of the yeast octameric rod. | Communications Biology

Fig. 4: AlphaFold assisted assembly of the yeast octameric rod.

From: The structure of a tetrameric septin complex reveals a hydrophobic element essential for NC-interface integrity

Fig. 4

A Structural model of the predicted Cdc10-Cdc10 NC-interface. Positions of the residues selected for disulfide bond engineering are indicated. B Disulfide bond engineering leads to crosslinking in oxidizing (o) but not in reducing (r) conditions. Double (marked with an arrowhead) and single (marked with a circle) crosslinks entail different packing density of the backbone chains for the Q21C/Q281C and A132C/L300C mutants. Non-crosslinked Cdc10 is marked with an asterisk. Shown is a Western blot detecting the S-tag fused to Cdc10 in an octameric septin rod preparation. C Structural model of an Shs1 containing octameric septin rod assembled from the tetrameric crystal structure and the predicted Cdc10-Cdc10 interface (dark-gray).

Back to article page