Fig. 1: Knockout of B7-H3 reduces clonogenic growth and tumorigenic signaling in gynecological cancer cells. | Communications Biology

Fig. 1: Knockout of B7-H3 reduces clonogenic growth and tumorigenic signaling in gynecological cancer cells.

From: Dimerization of the 4Ig isoform of B7-H3 in tumor cells mediates enhanced proliferation and tumorigenic signaling

Fig. 1

a 2000 HeLa wildtype (Clone 20) or CD276 knockout cells (Clone 6) were seeded and allowed to grow for 10 days, changing the media every 72 h. b HeLa CD276-/- cells (Clone 6) engineered to re-express 4Ig-B7-H3 (LV B7-H3) or a negative vector control (LV Neg) were used to perform a clonogenic assay as described above. 2,000 cells were seeded and allowed to grow for 10 days, changing the media every 72 h. c 2,000 SKOv3-ip-FLuc wildtype (Clone 22) or CD276 knockout cells (Clone 11) were seeded and allowed to grow for 10 days, changing the media every 72 h. a-c Clonogenic growth was quantified using ImageJ and represented in the bar graph (mean ± SD). The experiment was performed in technical triplicate with three biological replicates, ***p < 0.001 as determined by Student’s two-tailed t-test. d Reverse phase protein lysate array analysis and heat map visualization of the median normalized expression of each protein with and without 4Ig-B7-H3 expression for SKOv3-ip and HeLa cell lines. e Difference (KO-WT) in normalized protein expression for a subset of concordant proteins between SKOv3-ip and HeLa cell lines. f. X-Y plot of -Log(P values) and number of genes identified in each GO Term enrichment analysis with key pathway enrichment annotated.

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