Fig. 6: GPR116 deficiency affects islet number, insulin-positive area, and insulin expression.

a, b Immunofluorescence staining of mouse pancreatic islets of wt (a) and ko (b) mice. Shown are representative images of wt and ko islets with insulin-positive cells (green), somatostatin-positive cells (red), and DAPI-stained nuclei (blue). c Beta-cell amount was calculated as percentage of insulin-positive cell area relative to the total pancreatic islet area in pancreatic sections of wt and GPR116 ko mice. Shown is the mean ± SEM of all sections of five wt and ko mice. d The islet area was quantified in all analyzed sections of five wt and ko BALB/c animals. Data is given as mean ± SEM. e The total number of islets was counted in all pancreatic sections and is shown as mean ± SD of n = 5 per genotype. f The diameter of stained islets was calculated to classify islet size. Number of islets (<100 µm) were counted and are displayed as mean ± SD of all sections of n = 5 per genotype. g, h Insulin expression (Ins1 and Ins2) was determined in adult islets from 5 wt and ko animals. Data is given as percentage of wt expression (mean ± SEM) normalized to expression of Actb as reference gene. i, j Insulin expression (Ins1 and Ins2) was determined in pancreas of P1 animals (n = 5 per genotype). Data is given as percentage of wt expression (mean ± SEM) normalized to expression of Actb as reference gene. Statistical significance was tested using a two-tailed unpaired t test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001).