Fig. 6: Mature neudesin-deficient mice exhibit symptoms of anemia.

a, b Blood cell counts of 24-week-old WT and neudesin KO male mice. RBC, red blood cell count (WT: n = 16, KO: n = 15, P = 0.0193); Hb, hemoglobin (WT: n = 13, KO: n = 12, P = 0.0055); Ht, hematocrit (WT: n = 13, KO: n = 12, P = 0.0016); MCV, mean corpuscular volume (WT: n = 13, KO: n = 12, P = 0.6175); MCH, mean corpuscular hemoglobin (WT: n = 13, KO: n = 12, P = 0.5218); MCHC, mean corpuscular hemoglobin concentration (WT: n = 13, KO: n = 12, P = 0.4683); WBC, white blood cell count (WT: n = 6, KO: n = 5, P = 0.4938); PLT, platelet count (WT: n = 13, KO: n = 12, P = 0.3715). c Serum concentration of erythropoietin (Epo, WT: n = 12, KO: n = 13, P = 0.2198) and total bilirubin (WT: n = 11, KO: n = 9, P = 0.6419) from 24-week-old WT and neudesin KO male mice. d Spleens of neudesin KO male mice were significantly heavier than those of 24-weeks-old WT male mice (WT: n = 13, KO: n = 12, P = 0.0494). e Splenocyte suspensions from 24-week-old WT and neudesin KO male mice were assessed by flow cytometric analysis, and cell numbers of TER119⁺ red blood cells (WT: n = 13, KO: n = 12, P = 0.2580) and CD45⁺ white blood cells (WT: n = 13, KO: n = 12, P = 0.3981) in the spleen were determined. f Chart showing the number of RPMs from 24-week-old WT and neudesin KO male mice (WT: n = 13, KO: n = 12, P = 0.4752). g Mean fluorescence intensity (MFI) of surface macrophage receptors on RPMs from 24-week-old WT and neudesin KO male mice was measured by flow cytometry (WT: n = 13, KO: n = 12, FcγR1: P = 0.6545, FcγR2/3: P = 0.3510, FcγR4: P = 0.0027). *P < 0.05, **P < 0.05, and ***P < 0.001 by unpaired t-test. Each symbol represents an individual mouse. Data are shown as means ± SD of two or more experiments.