Fig. 2: SCL-12 accumulates in the gut during the dauer state where it sequesters cholesterol. | Communications Biology

Fig. 2: SCL-12 accumulates in the gut during the dauer state where it sequesters cholesterol.

From: Mobilization of cholesterol induces the transition from quiescence to growth in Caenorhabditis elegans through steroid hormone and mTOR signaling

Fig. 2

a SCL-12::mScarlet reporter dauer larva, whole animal (top), head region (bottom), posterior (post), and anterior (ant) directions are marked. Scale bar top: 50 µm, bottom: 25 µm. b SCL-12::mScarlet;daf-2 reporter dauer (0 h) and while exiting dauer at 6, 12, and 24 h after temperature switch to 15 °C. 12 h and 24 h insets are brightness adjusted images. Scale bar: 20 µm. c Mean fluorescence intensity of SCL-12::mScarlet;daf-2 reporter dauers (0 h) and while exiting dauer after temperature switch to 15 °C. Black: Fluorescence in the gut lumen. Red: Fluorescence outside of the lumen. Means and individual values of at least 14 individual worms (48 h: 7 worms). d SCL-12::mScarlet reporter animals (SCL-12::mSc; magenta) and fluorescent TopFluor cholesterol (green) in the gut of a dauer larva (0 h; left panels), 12 and 24 h after induction of dauer exit (middle and right panels). Merged channels show a co-localization of both fluorophores. Scale bars: 10 µm. e daf-2 and scl-12&13;daf-2 dauers fed with TopFluor cholesterol prior to entering the dauer state, representative images. Scale bar: 15 µm. f Mean fluorescence intensity of TopFluor cholesterol in the gut lumen from E, daf-2 (black) and scl-12&13;daf-2 (gray), and a single-mutant scl-12;daf-2 (white). Mean of 28 individual worms, unpaired two-tailed t-test with Welch’s correction showed p < 0.0001 between daf-2 and scl-12&13;daf-2 and p = 0.08 between daf-2 and scl-12;daf-2.

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