Fig. 4: Cholesterol is transported from the gut lumen to intestinal lysosomes with SCL-12 and then further to the epidermal region. | Communications Biology

Fig. 4: Cholesterol is transported from the gut lumen to intestinal lysosomes with SCL-12 and then further to the epidermal region.

From: Mobilization of cholesterol induces the transition from quiescence to growth in Caenorhabditis elegans through steroid hormone and mTOR signaling

Fig. 4

a daf-2 dauers co-labeled with TopFluor cholesterol (green) and Lysotracker dye (magenta; top panels), and 24 and 48 h after induction of dauer exit via temperature switch. Scale bar: 15 µm. The profile (fluorescence intensity in space) of both fluorophores in intestinal cells is similar at 24 h of dauer exit, indicating co-localization. b LMP-1::GFP;SCL-12::mScarlet;daf-2 dauers (top panels) and 24 h after induction of dauer exit (bottom panels). Scale bar: 10 µm. Lysosomal LMP-1::GFP (green) and SCL-12::mScarlet (magenta) show a similar profile at 24 h of dauer exit, indicating co-localization.

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