Fig. 5: Intact lysosomes are essential for dauer exit.

a Growth/length in mm in daf-2 exiting dauers induced by temperature switch to 15 °C on RNAis against factors of the apical trafficking and endocytic/lysosomal pathways, rab-7 (orange), cav-2 (green), rme-1 (blue), and glo-3 (purple) compared with mock treatment (EV: empty vector L4440; black). Average and individual values of 1 experiment with a minimum of 15 worms per condition, two-way ANOVA with post hoc Sidak’s multiple comparisons test comparing EV versus rab-7 showed p < 0.0001 and significant difference between the groups at all time points, EV versus cav-2 only 48 h was significantly different between the groups (p < 0.0001), and EV versus glo-3 only 96 h was significantly different between the groups (p = 0.0016). b Representing images of dauers of the lysosomal reporter LMP-1::GFP;daf-2 treated with EV and rab-7 RNAi. c Growth/length in mm in daf-2 exiting dauers on EV and rab-7 RNAi, treated with 200 nM DA (red/light orange), compared to solvent control (black/orange). Average and individual values of 1 experiment with a minimum of 25 worms per condition, two-way ANOVA with post hoc Tukey’s test comparing EV vs. rab-7 showed p < 0.0001, EV vs. rab-7 + DA p < 0.0001. rab-7 vs. rab-7 + DA was not significant. d Growth/length in mm in daf-2 exiting dauers on RNAi against rab-7 compared to EV, treated with 1 mM cholesterol (high cholesterol/HC; turquoise), compared to standard growing conditions that comprise 13 µM cholesterol (black/orange). Individual values of three independent experiments with a minimum of 30 individual worms per condition, two-way ANOVA with post hoc Tukey’s test comparing EV vs. rab-7 showed p = 0.0056, EV vs. rab-7 HC p = 0.0363, and rab-7 vs. rab-7 HC p = 0.002.