Fig. 5: The vesicle population at the distal seminal receptacle changes post-mating.

a, b A cross section of FIB-SEM image at the apical zone showing vesicles dispersed through the indicated zone in virgin (a) but densely localize at the apical zone after mating (b), white arrow heads; f, fibers at the intima layer; scale bar = 1 µm. Segmented 3D models of vesicles present in virgin (c) and mated (d) distal seminal receptacles; pink and purple indicate different ranges of vesicle sizes. e percentage of vesicles at a particular range of vesicle sizes in virgin (light gray) and mated (dark gray) distal seminal receptacle. Chi-Square test, p < 0.0001. See also Supplementary Video 6.