Fig. 3: HNF4A ectopic expression causes morphological changes in mouse fibroblast cells.
From: HNF4A guides the MLL4 complex to establish and maintain H3K4me1 at gene regulatory elements
a Schematic overview of the experimental setup to produce 3T3 cells with ectopic expression (EE) of HNF4A. b RT-qPCR confirming increased Hnf4a expression 48, 72 and 96 h after transduction. Error bars, standard deviation. n = 3 technical replicates. c Western blotting confirms protein presence of HNF4A at 72 h post transduction. d GFP verifies HNF4A expression and bright field imaging demonstrates the morphological shift from mesenchymal to epithelial cells. Scale bar, 50 µm. e Bar plot with RNA-seq Log2 ratio of FPKM values associated with epithelial (green) and mesenchymal genes (blue) from control and EE cells.
