Fig. 2: SAXS data from OaPAC subjected to size-exclusion (SEC-SAXS). | Communications Biology

Fig. 2: SAXS data from OaPAC subjected to size-exclusion (SEC-SAXS).

From: ATP-dependent conformational dynamics in a photoactivated adenylate cyclase revealed by fluorescence spectroscopy and small-angle X-ray scattering

Fig. 2: SAXS data from OaPAC subjected to size-exclusion (SEC-SAXS).The alternative text for this image may have been generated using AI.

(i) UV-trace (λ = 280 nm) of the eluting OaPAC in the SEC-SAXS experiment built by CHROMIXS, showing integrated intensities (light blue) versus time (frame number) for a SEC-SAXS run on OaPAC. Superimposed is the Rg-estimate for individual SAXS data-frames. The frames corresponding to the buffer (red dots) and sample (green dots) are automatically identified, averaged and subtracted one from another. (ii) X-ray scattering pattern of eluted OaPAC. Inset: Guinier plot of the data (iii) Dimensionless Kratky plot of OaPAC. (iv) Pair distribution function P(r) of OaPAC. Ab initio electron density envelope model (gray) based on the SAXS data is superimposed on the crystal structure of OaPAC (pdb:4yut).

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