Table 1 Basic SAXS parameters Rg (radius of gyration), Dmax. (maximum size) and Rg(GNOM) (radius of gyration calculated using GNOM) derived from the SAXS profiles of the titration series of OaPAC with ATP (Fig. 3).

From: ATP-dependent conformational dynamics in a photoactivated adenylate cyclase revealed by fluorescence spectroscopy and small-angle X-ray scattering

[ATPfinal]

Molar ratio (ATP:OaPAC)

Rg(Å) (Guinier) /r2 (fit)

ΔRg(Å) (Guinier)

Rg(Å) (GNOM*)

ΔRg(Å) (GNOM)

Dmax (Å) /χ2

0 μΜ

–

30.63 ± 0.31 /0.9790

–

30.65 ± 0.02

–

90 /1.0281

10 μΜ

0.14

31.54 ± 0.48 /0.9873

0.91

30.44 ± 0.02

−0.21

92 /1.0556

50 μΜ

0.71

31.84 ± 0.67 /0.9857

1.21

31.04 ± 0.04

0.39

105 /1.2875

70 μΜ

1.0

33.57 ± 0.41 /0.9921

2.94

33.21 ± 0.05

2.56

120 /1.2590

100 μΜ

1.4

33.95± 0.62 /0.9902

3.32

32.81 ± 0.05

2.16

126 /1.0839

150 μΜ

2.1

34.12 ± 0.39 /0.9957

3.49

33.94 ± 0.05

3.29

128 /1.0093

200 μΜ

2.9

34.90 ± 0.38 /0.9937

4.27

34.31 ± 0.06

3.66

130 /1.0808

250 μΜ

3.6

34.70 ± 0.61 /0.9903

4.07

34.28 ± 0.05

3.63

131 /1.0616

300 μΜ

4.3

34.93 ± 0.38 /0.9906

4.3

34.02 ± 0.06

3.37

131 /1.1655

400 μΜ

5.7

34.82 ± 0.50 /0.9930

4.19

34.43 ± 0.05

3.78

130 /1.0904

500 μΜ

7.1

35.01 ± 0.66 /0.9888

4.38

34.16 ± 0.05

3.51

130 /1.1407

700 μΜ

10.0

35.25 ± 0.65 /0.9867

4.62

33.93 ± 0.06

3.28

132 /1.1421

800 μΜ

11.4

35.23 ± 0.45 /0.9913

4.6

33.67 ± 0.05

3.02

131 /1.1337

1000 μΜ

14.3

35.91 ± 0.41 /0.9817

5.28

33.35 ± 0.05

2.7

131 /1.4938

  1. GNOM22 is an indirect transform program for small-angle scattering data processing. It reads in one-dimensional scattering curves (possibly smeared with instrumental distortions) and evaluates the particle distance distribution function P(r). χ2 is the chi square. All residual plots are presented in Supplementary Fig. 4 (Supplementary Note 4).
Back to article page