Fig. 2: Confinement does not increase cell migration of fibroblasts.

a Two-photon live imaging of a co-culture of cancer cells stained by membrane die FM 4-64 (red) and fibroblasts expressing GFP (green) at the onset of confluency. Time in hours, h. Scale bar: 50 μm. b Distribution of the instantaneous speed prior (blue bars) and after confluence (red bars). Reconstituted fibroblast 2D trajectories prior to confluence (blue line) and after confluence (red line). Time step: 20 min. Typical trajectory length: 15 h. c Path persistence prior to confluence (blue) and after confluence (red). Wilcoxon rank-sum test was performed (*p < 0.05). n = 38 cells.