Fig. 2: Tau binds DNA and nucleosomes.
From: Phosphorylation regulates tau’s phase separation behavior and interactions with chromatin
a EMSA of 20 nM 177 bp mononucleosomes and 147 bp ‘linker-free’ mononucleosomes with various concentrations of tau, in 20 mM HEPES buffer, 10 mM NaCl, 0.5 mM TCEP, 0.1% Tween, pH 7.2. b EMSA of 20 nM 177 bp DNA with various concentrations of tau, in the same low salt buffer. c Quantification of binding propensity based on the intensity of the unbound DNA or mononucleosome bands in Fig. 2a, b. Individual replicates are shown, and curves were fitted to a one-site-specific binding with Hill Slope equation. 177 bp DNA, 147 bp mononucleosomes, and 177 bp mononucleosomes were quantified for the following data points: 0, 125, 250, 500, and 1000 nM. 16–62 nM data points were excluded for the mononucleosome binding due to the smearing and complex bands overlapping with the unbound probe. MN – mononucleosomes.
