Fig. 3: Overexpression LAL disrupted the superior salinity tolerance in lal-1. | Communications Biology

Fig. 3: Overexpression LAL disrupted the superior salinity tolerance in lal-1.

From: The long noncoding RNA LAL contributes to salinity tolerance by modulating LHCB1s’ expression in Medicago truncatula

Fig. 3

a The scheme of LAL and MtLHCB1.1, MtLHCB1.2, MtLHCB1.3 and MtLHCB1.4 genes structure and Tnt1 insertion in the gene of lal-1. Empty boxes represent MtLHCB1s, and black boxes represent LAL. One arrow indicates the position where Tnt1 inserts in LAL. Bar = 400 bp. b The expression level of LAL in WT, lal-1 and lal-1;35 S:LAL#1 by qRT-PCR. c The plant survival rates measured after the four-week treatment of salinity. Error bars represent the SD from three biological replicates (grey dots). Columns labeled with asterisks indicate significant differences from those in WT (*P < 0.05, student’s t test). d Four-week-old plants of WT, lal-1 and lal-1;35 S:LAL#1 plants after a four-week successive exposure to 50, 100, 150 and 200 mM NaCl. Bar = 5 cm. e The MDA content of WT, WT, lal-1 and lal-1;35 S:LAL#1. The expression level of MtRbohB (f), MtRbohD (g), MtCAT1 (h), MtABA1 (i) and MtNCED3 (j) in WT, lal-1 and lal-1;35 S:LAL#1 by qRT-PCR. The expression level of MtLHCB1.1, MtLHCB1.2, MtLHCB1.3, and MtLHCB1.4 (k) in WT, lal-1 and lal-1;35 S:LAL#1 by qRT-PCR. MtUBIQUITIN was used as the internal control. The expression level in WT was set at 1.0. Error bars represent the SD from three biological replicates (grey dots). Columns labeled with asterisks indicate significant differences from those in WT (*P < 0.05, student’s t test). n = 3.

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