Fig. 1: PF4 interaction with a fixed-length (twenty saccharide unit-long) heparin fragment. | Communications Biology

Fig. 1: PF4 interaction with a fixed-length (twenty saccharide unit-long) heparin fragment.

From: Molecular architecture and platelet-activating properties of small immune complexes assembled on heparin and platelet factor 4

Fig. 1

A Native MS of PF4/dp20 mixtures (top: 0.27 and 0.028 mg/mL, respectively; bottom: 0.27 and 0.045 mg/mL). The charge ladders shown in maroon and red were obtained by limited charge reduction of ionic populations at m/z 3,723 (top) and 3,953 (bottom). The two masses calculated based on these charge ladders correspond to 1:1 (37.2 kDa) and 1:2 (43.6 kDa) protein:heparinoid stoichiometries. B Molecular modeling of PF4/dp20 interactions showing 1:1 and 1:2 protein/heparinoid complexes. The iso-potential surface (6kT/e) represents the positive belt around the protein, while the heparin chain is shown using a space-fill model (without the corresponding iso-potential surface).

Back to article page