Table 2 Primers used in this study

From: Emergence and clonal expansion of a qacA-harbouring sequence type 45 lineage of methicillin-resistant Staphylococcus aureus

Primer

Sequence (5’ – > 3’)

Description

qacA_Fp

CCTCACTAAAGGGAACAAAAGCTGGGTACCCTTTTAATTCTAGCGTGCCTAC

Flanking primers used for amplification of gene cassettes for qacA deletion and complementation

qacA_Rp

CGACTCACTATAGGGCGAATTGGAGCTCCGTAATTTAGAAATAATATTTATTGGTATTTCAAG

qacA_SOE_Fp

CTAATCTACAATATCTAAAAATATATGTTTAGTATTAAGTTCCCTCCAATCCTTATAG

Construction of gene cassette for qacA deletion by Splicing by Overlap Extension PCR

qacA_SOE_Rp

CTATAAGGATTGGAGGGAACTTAATACTAAACATATATTTTTAGATATTGTAGATTAG

qacA_comp_Fp

CTATTACAACCCACGGAATAATATCTGCTAGTC

Construction of gene cassette for qacA complementation by Splicing by Overlap Extension PCR

qacA_comp_Rp

GACTAGCAGATATTATTCCGTGGGTTGTAATAG

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