Fig. 7: CREBH directly regulated the DGAT1 promoter in large yellow croaker. | Communications Biology

Fig. 7: CREBH directly regulated the DGAT1 promoter in large yellow croaker.

From: Differences in diacylglycerol acyltransferases expression patterns and regulation cause distinct hepatic triglyceride deposition in fish

Fig. 7: CREBH directly regulated the DGAT1 promoter in large yellow croaker.

a Relative luciferase activities of the DGAT1 promoter in large yellow croaker in HEK-293T cells. The control group was co-transfected with pGL6-basic plasmid and pCS2+ empty plasmid. The luciferase activity in control group was selected as normalization (n = 4). CREBH-F: CREBH full length. CREBH-N: CREBH nucleus form. b Dual luciferase activity analysis of CREBH gradient concentration in the DGAT1 promoter in large yellow croaker (n = 4). c Effects of CREBH on the DGAT1 promoter with mutation in the CREBH binding site (n = 3). d cAMP stimulation enhanced activation effect of CREBH or CREBH-N on the DGAT1 promoter (n = 4). e ChIP analysis of CREBH binding to the DGAT1 promoter (1709bp~1720bp) in HEK-293T cells. Values represent mean ± SEM. *P < 0.05; **P < 0.01.

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