Fig. 5: Morphology and organization of cristae.

The distribution of total volumes of the intracristal space a and surface areas of mitochondrial cristae b in 3D are presented. Distributions of average crista half-widths d, cristae curvature/roughness e, and average half-distances between cristae across the matrix f in 3D are also shown. The distribution of volumes of the mitochondrial matrix is given in c. The correlations between cristae surface area (intracristal side) and intracristal volume are almost perfectly linear g. A positive linear relationship between the volumes of mitochondria and their intracristal spaces is also visible h. No clear relationship is detected between mitochondria volume and cristae width i or intercristal distance j. Please note that the counts and bin boundaries in histograms are computed on log-transformed values to better visualize the data, as the untransformed data are highly left skewed. For 32 out of 360 mitochondria, it was not possible to detect the inner mitochondria membrane. In subfigure h this results in the vertically scattered data points near the origin. Since the segmentation of these mitochondria does not seem to be erroneous, the data points are included in the scatter plot. The sample sizes vary for plots due to the differences in inclusion criteria for the parameters. CM crista membrane, FWHM full-width-half-maximum, mad median absolute deviation, std standard deviation.