Fig. 1: Long photoperiod induces an increase in gene expression and circulating sex steroids.

a Illustration showing the experimental design. Fish were raised from hatching in LP or SP for 4 months before the photoperiod was changed. Fish were sampled on day 0 (just before the photoperiod change), 7, 14, and 30. b–d The fluctuation of pituitary fshb and lhb expression as well as blood E2 levels observed in females at different time points following the photoperiod change. e–g The fluctuation of cyp19a1b levels in the pituitary as well as gnrh1 and kiss1 in the brain at different time points following the photoperiod change. The letters (a–c) show statistically differences between each time points and group. The statistical analyses were performed using non-parametric tests, Scheirer–Ray–Hare Test, followed by Dunn’s Test. All graphs are represented as mean ± SEM. Individual numbers (n) are annotated throughout the figure.