Fig. 3: Tsh indirectly stimulates gene expression of gonadotrope cells in SP females. | Communications Biology

Fig. 3: Tsh indirectly stimulates gene expression of gonadotrope cells in SP females.

From: Day length regulates gonadotrope proliferation and reproduction via an intra-pituitary pathway in the model vertebrate Oryzias latipes

Fig. 3

tshba (a) and tshbb (b) mRNA levels in LP and SP condition in female medaka. c Experimental scheme illustrating the treatment of 0.5 µM of bovine TSH pituitary extract to dissociated cell culture and organ culture of medaka pituitary to evaluate direct and indirect effect of Tsh on gene expression of gonadotrope cells. d–f Effect of TSH or vehicle (control) on fshb, lhb and aromatase (cyp19a1b) mRNA levels in dispersed pituitary cell cultures in female medaka from LP and SP condition (n = 3; in which each replicate represents 4 pooled pituitaries), the bar represents mean ± SEM. g–h Effect of TSH or vehicle (control) on fshb and lhb mRNA levels in ex vivo medaka pituitary organ cultures of female medaka from LP and SP condition. The statistical analyses were performed using two-sample independent t-test (b, d–f) or Mann–Whitney U test (a, g, h). All graphs (unless otherwise stated) are represented as mean ± SEM with the jitter dots representing each individual (p-values: *< 0.05; **< 0.01). Individual numbers (n) are annotated throughout the figure.

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