Fig. 4: Tsh stimulates mitotic activity in the pituitary of SP females. | Communications Biology

Fig. 4: Tsh stimulates mitotic activity in the pituitary of SP females.

From: Day length regulates gonadotrope proliferation and reproduction via an intra-pituitary pathway in the model vertebrate Oryzias latipes

Fig. 4

a Effect of 0.5 µM of bovine TSH pituitary extract for 24 h on mki67 levels in the female medaka pituitary organ cultures in LP and SP condition. b Schematic illustration of ex vivo medaka pituitary organ culture with bovine TSH pituitary extract and BrdU treatment to evaluate gonadotrope mitosis. c Number of mitotic cells in SP females exposed to TSH for 12 h. d Visual representation from the pituitary of dTg fish with Gfp signal for Lh cell (cyan), Fshβ for Fsh cells (magenta), and BrdU for mitotic cells (yellow) in the pituitary of SP females treated with TSH, as taken by Thunder fluorescence microscope. The statistical analyses were performed using two-sample independent t-test, in which the graph represents mean ± SEM while the jittered dots represent each individual (p-values: *< 0.05; **< 0.01). Individual numbers (n) are annotated throughout the figure. Arrows point to the mitotic cells. Scale bar: 20 µm.

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