Table 2 Primer sequences used for mRNA level analysis in the medaka pituitary
Gene | Sequence (5’ - 3’) | Accession Number | PCR product size (bp) | Efficiency | Reference |
|---|---|---|---|---|---|
rpl7 | F: TGCTTTGGTGGAGAAAGCTC | NM_001104870 | 98 | 2.03 | |
R: TGGCAGGCTTGAAGTTCTTT | |||||
gapdh | F: CCTCCATCTTTGATGCTGGT | XM_004077972.3 | 170 | 2.01 | |
R: ACGGTTGCTGTAGCCAAACT | |||||
lhb | F: CCACTGCCTTACCAAGGACC | NM_001137653.2 | 100 | 2 | |
R: AGGAAGCTCAAATGTCTTGTAG | |||||
fshb | F: GACGGTGCTACCATGAGGAT | NM_001309017.1 | 73 | 2.03 | |
R: TCCCCACTGCAGATCTTTTC | |||||
tshba | F: ATGTGGAGAAGCCAGAATGC | XM_004068796.4 | 88 | 2 | |
R: CTCATGTTGCTGTCCCTTGA | |||||
tshbb | F: CCCAGTGCATTGCTATCAACAC | XM_011477157.2 | 79 | 2 | This study |
R: CCAAACCGGCCTCTAAAATTGG | |||||
mki67 | F: ACCAATCTGAGCACAGCCAAC | XM_011487928.3 | – | 2 | |
R: GGTGCAGGTGGATACTCAAAC | |||||
cyp19a1b | F: AAGAAGATGATCCAGCAAGAG | XM_011475856.1 | 88 | 2 | |
R: AGCATCAGAAGAAGTAAGAAAAGTG |
