Fig. 3: Multicolor imaging of specific types of cells in different tissues of transgenic mice co-expressing UFObow and Cre. | Communications Biology

Fig. 3: Multicolor imaging of specific types of cells in different tissues of transgenic mice co-expressing UFObow and Cre.

From: UFObow: A single-wavelength excitable Brainbow for simultaneous multicolor ex-vivo and in-vivo imaging of mammalian cells

Fig. 3: Multicolor imaging of specific types of cells in different tissues of transgenic mice co-expressing UFObow and Cre.

Each panel consists of three fluorescence images: a tissue slice with FP fluorescence (top), and a region of interest (ROI, white box) of the slice with FP fluorescence (bottom left) and immunofluorescence (bottom right). a, b The representative fluorescence images of brain and lymph node slices of the CX3CR1cre:UFObow mouse. The PE anti-CX3CR1 antibody labeled CX3CR1-positive cells. Scale bar in the large image of a, 100 μm. Scale bar in the large image of b, 200 μm. Scale bar in small images, 50 μm. c The representative fluorescence images of a spleen slice of the CD11ccre: UFObow mouse. The Alexa Fluor 647 anti-CD11c antibody labeled CD11c-positive cells. Scale bar in the large image, 100 μm. Scale bar in small images, 50 μm. d The representative fluorescence images of a brain slice of the Chatcre:UFObow mouse. The Alexa Fluor 647 anti-VAChT antibody labeled VAChT-positive cells. Scale bar in the large image, 500 μm. Scale bar in small images, 50 μm.

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