Fig. 6: KAT5 acetylates STUB1 to relieve OGD/R-induced cardiomyocyte injury via LATS2/YAP/β-catenin axis. | Communications Biology

Fig. 6: KAT5 acetylates STUB1 to relieve OGD/R-induced cardiomyocyte injury via LATS2/YAP/β-catenin axis.

From: STUB1 is acetylated by KAT5 and alleviates myocardial ischemia-reperfusion injury through LATS2-YAP-β-catenin axis

Fig. 6

a Bioinformatic analysis predicted the potential binding sites (BS) of KAT5 to STUB1 promoter. The direct binding of KAT5 to STUB1 promoter was validated by ChIP (b) and dual-luciferase reporter assay (c). d The direct binding of KAT5/H3K27ac to STUB1 promoter in KAT5-silenced AC16 and HL-1 cells was detected by ChIP assay. e ChIP-re-ChIP assay validated the interaction of KAT5 with H3K27ac on the STUB1 promoter. AC16 and HL-1 cells were transfected with shKAT5, STUB1 overexpression lentivirus, or a combination of them, followed by stimulation with OGD/R. f Cell viability was detected by CCK-8. Data represent the mean ± SD. n = 3 independent experiments. b–d Student’s t test was used for statistical analysis. e, f One-way ANOVA followed by Bonferroni was performed for statistical analysis. Box plots represent median with minimum and maximum whiskers. *p < 0.05, **p < 0.01, ***p < 0.001 versus indicated group.

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