Fig. 3: Pseudotime trajectory analysis and dynamic changes of epithelial cell during development and aging.

a Pseudotime trajectory of alveoli epithelial cells inferred using monocle2 method. b Heatmap showing differentially expressed genes (DEGs) alone with the pseudotime as in (a), catalogs into 4 gene module clusters (left). Gene ontology terms enriched for each gene module clusters (right). c Gene ontology enrichment analysis of gene expression in cluster 1 of ciliated cell (development). d Gene ontology enrichment analysis of gene expression in cluster 6 of ciliated cell (aging). e Gene ontology enrichment analysis of up (top part, red)/down (bottom part, blue)-regulated gene expression in aging ciliated cell. f Gene ontology enrichment analysis of gene expression in cluster 2 of basal cell (development). g Gene ontology enrichment analysis of gene expression in cluster 6 of basal cell (aging). h Gene ontology terms of up (top part, red)/down (bottom part, blue)-regulated gene expression in aging basal cell. i Gene ontology enrichment analysis of gene expression in cluster 1 of club cell (aging). j Gene ontology enrichment analysis of gene expression in cluster 6 of club cell (aging). k Gene ontology terms of up (top part, red)/down (bottom part, blue)-regulated gene expression in aging club cell.