Fig. 1: OmpX + LTA1 immunization promotes Il1r1 transcript expression in CD4 T cells and induces IL-1 and other proinflammatory cytokines in vivo.
From: Vaccine-elicited IL-1R signaling results in Th17 TRM-mediated immunity
a Representative UMAP plot of Il1r1 expression in CD4+ cells from naïve spleens and OmpX+LTA1 immunized lungs. b The Log2 expression levels of Il1r1 from lung CD4+ T cells (lung) and naïve splenic CD4+ T cells (spleen) shown in a. c Representative flow cytometry histograms showing IL-1R1 on CD4+ T cells (CD45+, CD19-, CD3+, CD4+) from single cell lung suspensions of PBS treated and OmpX+LTA1 vaccinated mice. Concentrations of (d) GM-CSF, (e) IL-17A, (f) IL-6, (g) IL-27, (h) IL-10, (i) MCP-1, (j) IL-12p70, (k) IFN-β, (l) IFNγ, (m) TNF, (n) IL-1α, and (o) IL-1β in the serum, BAL fluid, and lung homogenate of immunized and unimmunized mice. Data are presented as the mean +/− SEM, n = 3 mice per group. Data were analyzed using the unpaired T test.
