Fig. 4: Transgenes of truncated NIA1 and NIA2 fragments effectively induce both gene silencing.
From: Multiple factors and features dictate the selective production of ct-siRNA in Arabidopsis
a Schematic illustration of the consecutive truncated 600-nt NIA1 and NIA2 fragments. b GFP fluorescence in transgenic plants expressing truncated NIA1 and NIA2 fragments. Scale bar = 100 μm. c Fluorescence intensity was detected in transgenic plants. d Abundance of 21-nt and 22-nt ct-siRNAs (TPM, tags per million) accumulated at NIA1 and NIA2 in transgenic plants. n = 2 biologically independent samples. FL, full-length. e Northern blotting of ct-siRNAs produced from GFP, NIA1, and NIA2 in transgenic plants. f Distribution of 21-nt and 22-nt ct-siRNAs generated from NIA1 and NIA2 in transgenic plants. g Sequence similarity between truncated NIA1 and NIA2 fragments. h GC contents of full-length and truncated NIA1 and NIA2 fragments.
