Fig. 1: EPI-MES and Ca2+ signaling genes are cell geometry dependent in MCF-7 epithelial-like breast cancer cells.
From: Cellular geometry and epithelial-mesenchymal plasticity intersect with PIEZO1 in breast cancer cells
a MCF-7 cells on unpatterned surfaces, b 1100 μm2 “O” (top) and “L” (bottom) micropatterns, and c 700 μm2 “O” (top) and “L” (bottom) micropatterns. Scale bar = 50 μm. d Proportion of occupied micropatterns with single cells. Data from four individual microplate wells, two independent cell platings: 1100 μm2 “O” micropatterns, n = 454 micropatterns; “L” micropatterns, n = 390 micropatterns (top); 700 μm2 “O” micropatterns, n = 508 micropatterns; “L” micropatterns, n = 454 micropatterns (bottom). e Heatmap of expression changes (-ΔΔCt) including EPI-MES markers, Ca2+ channels and Ca2+ channel regulators, and other genes. Data represent the average gene expression changes normalized to unpatterned wells (-ΔΔCt) (n = 4 biological replicates, except TRPV1 n = 3 biological replicates). Unmarked P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 (“O” vs “L”).
