Fig. 4: Verifying the expression of miR-455-3p, TCONS_00039830 and Smad2 in rats with MASLD and both MASLD and OSA. | Communications Biology

Fig. 4: Verifying the expression of miR-455-3p, TCONS_00039830 and Smad2 in rats with MASLD and both MASLD and OSA.

From: Adipocyte-derived exosomes from obstructive sleep apnoea rats aggravate MASLD by TCONS_00039830/miR-455-3p/Smad2 axis

Fig. 4

a HE staining and oil red O staining of liver tissues of rats in Control, MASLD, and MASLD + OSA groups, as well as NAFLD activity score (NAS); arrows point out histopathological changes; b Triglyceride level of rat serum in Control, MASLD, and MASLD + OSA groups; c Cholesterol level of rat serum in Control, MASLD, and MASLD + OSA groups; d Liver weight of rats in Control, MASLD, and MASLD + OSA groups; e Aspartate aminotransferase (ALT) and alanine aminotransferase (AST) levels of rat serum in Control, MASLD, and MASLD + OSA groups; f The relative expression of TCONS-00039830 in liver tissues of rats in Control, MASLD, and MASLD + OSA groups detected by RT–qPCR; g The relative expression of miR-455-3p in liver tissues of rats in Control, MASLD, and MASLD + OSA groups assessed by RT–qPCR; h The relative mRNA expression of Smad2 in liver tissues of rats in Control, MASLD, and MASLD + OSA groups measured by RT–qPCR; i The relative protein expression of Smad2 in liver tissues of rats in Control, MASLD, and MASLD + OSA groups detected by Western blotting, as well as the quantification results. n = 6 rats/group; *P < 0.05, **P < 0.01, ***P < 0.001 versus the Control group; ###P < 0.001 versus the MASLD group; MASLD Metabolic Dysfunction-associated Steatotic Liver Disease, OSA Obstructive Sleep Apnoea. x̅ ± SD.

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