Fig. 7: Exosomes derived from rats with OSA aggravates hepatic steatosis in MASLD. | Communications Biology

Fig. 7: Exosomes derived from rats with OSA aggravates hepatic steatosis in MASLD.

From: Adipocyte-derived exosomes from obstructive sleep apnoea rats aggravate MASLD by TCONS_00039830/miR-455-3p/Smad2 axis

Fig. 7: Exosomes derived from rats with OSA aggravates hepatic steatosis in MASLD.

a The expression of the exosome marker proteins CD9, CD63 and Cyto C in adipocytes, exosomes, and OSA-exosomes detected by Western blotting; b Observation of extracted exosomes via transmission electron microscope; c The size of exosomes was detected by the particle size analyser; d Intracellular triglycerides in hepatocytes in Control, FFA, FFA + OSA, FFA + Exo, FFA + OSA-Exo groups; e Intracellular cholesterol in hepatocytes in Control, FFA, FFA + OSA, FFA + Exo, FFA + OSA-Exo groups; f Fat accumulation in hepatocytes in Control, FFA, FFA + OSA, FFA + Exo, FFA + OSA-Exo detected by oil red O staining; g Hepatic steatosis status in hepatocytes in Control, FFA, FFA + OSA, FFA + Exo, FFA + OSA-Exo groups detected by Bodipy staining, as well as the quantification result. **P < 0.01, ***P < 0.001 versus Control group; #P < 0.05, ##P < 0.01, ###P < 0.001 versus FFA group; OSA Obstructive Sleep Apnoea; FFA Free Fatty Acid. x̅ ± SD.

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