Fig. 8: TCONS_00039830 mediates hepatic steatosis induced by miR-455-3p/Smad2 through exosomes derived from rats with OSA. | Communications Biology

Fig. 8: TCONS_00039830 mediates hepatic steatosis induced by miR-455-3p/Smad2 through exosomes derived from rats with OSA.

From: Adipocyte-derived exosomes from obstructive sleep apnoea rats aggravate MASLD by TCONS_00039830/miR-455-3p/Smad2 axis

Fig. 8

a The relative expression of TCONS_00039830 in hepatocytes in Control, FFA, FFA + OSA, FFA + Exo, and FFA + OSA-Exo groups detected by RT-qPCR; b The relative expression of TCONS_00039830 in adipocytes and exosomes from healthy rats and rats with OSA measured by RT-qPCR; c The relative expression of TCONS_00039830 in adipocyte-derived exosomes after transfection with si-TCONS_00039830 determined by RT-qPCR; d The relative expression of TCONS_00039830 in hepatocytes in Control, OSA and OSA + si-TCONS_00039830 groups assessed by RT-qPCR; e Intracellular triglyceride level in hepatocytes in the Control, FFA, FFA + OSA-Exo and FFA + OSA-siRNA-Exo groups; f Intracellular cholesterol level in hepatocytes in Control, FFA, FFA + OSA-Exo and FFA + OSA-siRNA-Exo groups; g Fat accumulation in hepatocytes detected via oil red O staining; h The relative expression of miR-455-3p in hepatocytes in Control, FFA, FFA + OSA-Exo and FFA + OSA-siRNA-Exo groups detected by RT-qPCR; i The relative mRNA and protein expressions of Smad2 in hepatocytes from the Control, FFA, FFA + OSA-Exo and FFA + OSA-siRNA-Exo groups assessed by RT-qPCR and Western blotting, as well as the quantification result. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control group; #P < 0.05, ## P < 0.01, ###P < 0.001 versus FFA group; ^P < 0.05, ^^P < 0.01 versus FFA + OSA-Exo group; OSA Obstructive Sleep Apnoea, FFA Free Fatty Acid. x̅ ± SD.

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