Fig. 1: Establishing a sensitized background for a deficiency screen.
From: hkb is required for DIP-α expression and target recognition in the Drosophila neuromuscular circuit
a Cartoon depicting the innervation pattern of dorsal Is MN and ventral Is MN. b Representative images of muscle 4 with Is innervation or without Is innervation, in trans-heterozygotes of DIP-α (the DIP-α-GAL4 is also a null allele) and dpr10. 51% of m4s are innervated by the dorsal Is MN. GFP (green), DLG (magenta) and HRP (blue) are shown in the images. Arrow pointing to the Is NMJ. c Workflow of the deficiency screen. Male flies carrying the deficiency chromosome were crossed to females with DIP-α and dpr10 mutations. Female third instar larvae were selected against the second and third chromosome balancers (CyO,actin > GFP and TM6,Tb,Hu). Triple-heterozygous larvae were dissected and Is innervation frequency on m4, 3, 12 and 13 were scored. Cartoon is created with BioRender.com.
