Fig. 5: hkb is required for DIP-α-EGFP expression in dorsal Is MNs.
From: hkb is required for DIP-α expression and target recognition in the Drosophila neuromuscular circuit
a Cartoon depicting the focal planes in (b–d). Representative images of dorsal Is MN cell bodies (arrows in b) and ventral Is MN cell bodies (arrows in b’) labeled with GFP (green), Eve (red), and FasII (magenta) in control embryos. DIP-α-EGFP is expressed in both dorsal and ventral Is MNs. Note that there are also two interneurons on the dorsal side of each hemisegment that express DIP-α-EGFP (arrowheads in b). c’ Representative images of dorsal Is MN cell bodies (dashed circles in c) and ventral Is MN cell bodies (arrows in c’) in hkb2 mutant embryos. Eve and DIP-α-EGFP are missing in dorsal Is MNs, whereas DIP-α-EGFP expression in ventral Is MNs is not affected. In addition, DIP-α-EGFP expressing interneurons are not affected (arrowheads in c), suggesting that Hkb function is specific to dorsal Is MNs. Representative images of dorsal Is MN cell bodies (dashed circles in d) and ventral Is MN cell bodies (arrows in d’) in heteroallelic hkb mutant embryos (hkb2 /hkbA321R1). Eve and DIP-α-EGFP are missing in dorsal Is MNs, whereas DIP-α-EGFP expression in ventral Is MNs is not affected. In addition, DIP-α-EGFP expressing interneurons are not affected (arrowheads in d), confirming that Hkb function is specific to dorsal Is MNs.
